Shrapnel from an airstrike on Monday killed a doctor and injured two paramedics working in a field hospital in the Al-Tweisha area south of Tripoli.
"Dr. Samer Sulaiman al-Sibai was killed and two paramedics in the Al--Zahra field hospital were wounded," the Health Ministry said in a statement.
The statement condemned the airstrike, describing it as "unjustified and brutal attack on the field hospital and its medical staff."
"Health workers, hospitals and paramedics in Libya are not a military target and not a party to armed conflicts," the statement said.
Medical workers had been attacked previously in Tripoli, despite warnings by the World Health Organization against attacking medical workers and hospitals.
The LNA has been leading a military campaign since early April to take over Tripoli, home to nearly 2 million people, from the government.
The fighting has claimed thousands of lives and injured many others, and also displaced nearly 120,000 people.